Introduction

This report presents a statistical analysis of behavioral data comparing experimental groups based on multiple factors. The statistical significance of differences between groups was assessed using an automated decision pipeline.

For continuous data, normality (Shapiro-Wilk test) and variance homogeneity (Fligner-Killeen test) were tested.

Based on the results, the pipeline automatically applied data transformation (log1p or square root) and selected either Parametric Tests (Welch’s t-test, 3-Way ANOVA, Tukey HSD) or Non-parametric Tests (Mann-Whitney U, Kruskal-Wallis, Conover-Holm).

For Rota-Rod evaluation an additional Survival Analysis was done to account for the artificial ceiling effect of RR test (maximum single trial duration of 300 seconds). Survival probabilities were estimated using the Kaplan-Meier method, and statistical differences between survival curves were assessed via the Log-Rank test. To systematically evaluate the interaction between multiple factors, a Cox Proportional Hazards Model was utilized, providing an assessment of the relative risk of falling.

Description of Behavioral Parameters

1. Rota-Rod Analysis

Latency to fall coefficient is the arithmetic mean of the highest results obtained by a given individual in all measurement days. Max single test duration: 300 sec/40 rpm.

2. Grip-Test Analysis

Peak force coefficient is the arithmetic mean of the highest results obtained by a given individual in all measurement days

3. Open-Field Analysis

Following behavioral variables analyzed in this report were computed using EthoVision XT video tracking software (Noldus Information Technology). The parameters are defined as follows:

3.1. General Locomotion and Zone Analysis
  • Total Distance [cm]: The total length of the path traveled by the subject’s center point (CP) during the trial.
  • Cumulative Duration in Zone [s]: The total amount of time the subject’s center point was detected within a specific user-defined zone (e.g., Center, Sides, Open Arms, Closed Arms).
    • Open Field (OF): Zones are defined as “Center” (central square) and “Sides” (periphery).
3.2 Mobility State Analysis

EthoVision XT determines mobility states based on changes in the subject’s detected surface area (pixel changes) between consecutive frames, independent of the center point’s spatial displacement. This allows for the detection of movement in general (e.g., grooming, struggling) even if the animal remains in the same location.

  • Immobile: The subject is considered immobile when the percentage of pixel change between frames is below the defined lower threshold (t=20%). This represents freezing or total lack of movement.
  • Mobile: The subject is considered mobile when the percentage of pixel change is between the lower (immobile) and upper (highly mobile) thresholds. This represents general activity (e.g., walking, exploration).
  • Highly Mobile: The subject is considered highly mobile when the percentage of pixel change exceeds the defined upper threshold (t=60%). This represents vigorous activity (e.g., running, jumping, strong struggling).
3.3 Rotations Analysis

Rotations are defined as the number of full 360° turns completed by the animal, analyzed in both Clockwise and Counter-Clockwise directions. For this analysis method of locomotive rotation was used to calculate number of rotation:

  • Locomotive Rotation: Calculated based on the cumulative change in the heading of the body point between consecutive samples. This method detects the animal walking in circles around the arena. Thresholds set: 50 deg minimal angle & 2 cm minimal distance.
  • Total number of rotations: is a sum of clockwise rotations and counter-clockwise rotations.

Analysis Results

In this section, a boxplot and tables with statistical test results are presented for each defined behavioral parameter from performed tests.

Rota-Rod Results:

Analysis for parameter: Latency to fall coefficient [s] (Central Tendencies)

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Raw data.
3. Selected pathway: PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw latency data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 121.080 123.542 43.571 33.160
Group 2 21 115.218 112.133 58.974 50.265
Sex female 23 118.931 122.320 48.065 35.086
Sex male 20 117.397 104.288 55.699 37.872
Genotype KO 18 102.863 103.773 46.790 40.350
Genotype WT 25 129.273 122.320 52.153 33.213
Factor ActvSub 22 121.535 115.402 44.326 36.535
Factor NaCl 21 114.742 104.380 58.326 46.758
Interaction KO-female-ActvSub 5 137.847 161.820 57.072 7.448
Interaction KO-female-NaCl 5 101.062 103.350 51.760 39.912
Interaction KO-male-ActvSub 4 93.813 92.915 16.541 12.656
Interaction KO-male-NaCl 4 70.432 65.072 25.700 15.223
Interaction WT-female-ActvSub 7 116.144 116.107 48.952 16.781
Interaction WT-female-NaCl 6 121.311 123.288 42.960 26.271
Interaction WT-male-ActvSub 6 132.713 136.923 39.181 32.421
Interaction WT-male-NaCl 6 149.112 143.543 77.430 46.987

1. Main comparisons

Summary of main comparisons

Test used: Welch Two Sample t-test

Comparison P-value
Group: 1 vs 2 0.7139846
Sex: male vs female 0.9240293
Genotype: WT vs KO 0.0897823
Factor: NaCl vs ActvSub 0.6707170

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: 3-Way ANOVA (Genotype x Sex x Mod)

Df Sum Sq Mean Sq F value Pr(>F)
Genotype 1 7299.5561 7299.5561 2.9097 0.0969
Sex 1 64.4637 64.4637 0.0257 0.8736
Mod 1 416.6082 416.6082 0.1661 0.6861
Genotype:Sex 1 9334.0124 9334.0124 3.7206 0.0619
Genotype:Mod 1 4479.1150 4479.1150 1.7854 0.1901
Sex:Mod 1 392.7935 392.7935 0.1566 0.6947
Genotype:Sex:Mod 1 3.0579 3.0579 0.0012 0.9723
Residuals 35 87805.1279 2508.7179 NA NA
Pairwise comparisons for interaction

Post-hoc used: Tukey HSD

Comparison P-value (Tukey)
KO-female-NaCl-KO-female-ActvSub KO-female-NaCl-KO-female-ActvSub 0.9375135
KO-male-ActvSub-KO-female-ActvSub KO-male-ActvSub-KO-female-ActvSub 0.8887144
KO-male-NaCl-KO-female-ActvSub KO-male-NaCl-KO-female-ActvSub 0.4921960
WT-female-ActvSub-KO-female-ActvSub WT-female-ActvSub-KO-female-ActvSub 0.9950132
WT-female-NaCl-KO-female-ActvSub WT-female-NaCl-KO-female-ActvSub 0.9992765
WT-male-ActvSub-KO-female-ActvSub WT-male-ActvSub-KO-female-ActvSub 0.9999997
WT-male-NaCl-KO-female-ActvSub WT-male-NaCl-KO-female-ActvSub 0.9999436
KO-male-ActvSub-KO-female-NaCl KO-male-ActvSub-KO-female-NaCl 0.9999986
KO-male-NaCl-KO-female-NaCl KO-male-NaCl-KO-female-NaCl 0.9829100
WT-female-ActvSub-KO-female-NaCl WT-female-ActvSub-KO-female-NaCl 0.9995060
WT-female-NaCl-KO-female-NaCl WT-female-NaCl-KO-female-NaCl 0.9973616
WT-male-ActvSub-KO-female-NaCl WT-male-ActvSub-KO-female-NaCl 0.9639973
WT-male-NaCl-KO-female-NaCl WT-male-NaCl-KO-female-NaCl 0.7561040
KO-male-NaCl-KO-male-ActvSub KO-male-NaCl-KO-male-ActvSub 0.9975410
WT-female-ActvSub-KO-male-ActvSub WT-female-ActvSub-KO-male-ActvSub 0.9960886
WT-female-NaCl-KO-male-ActvSub WT-female-NaCl-KO-male-ActvSub 0.9885446
WT-male-ActvSub-KO-male-ActvSub WT-male-ActvSub-KO-male-ActvSub 0.9255756
WT-male-NaCl-KO-male-ActvSub WT-male-NaCl-KO-male-ActvSub 0.6807295
WT-female-ActvSub-KO-male-NaCl WT-female-ActvSub-KO-male-NaCl 0.8245434
WT-female-NaCl-KO-male-NaCl WT-female-NaCl-KO-male-NaCl 0.7621127
WT-male-ActvSub-KO-male-NaCl WT-male-ActvSub-KO-male-NaCl 0.5430576
WT-male-NaCl-KO-male-NaCl WT-male-NaCl-KO-male-NaCl 0.2574163
WT-female-NaCl-WT-female-ActvSub WT-female-NaCl-WT-female-ActvSub 0.9999995
WT-male-ActvSub-WT-female-ActvSub WT-male-ActvSub-WT-female-ActvSub 0.9987337
WT-male-NaCl-WT-female-ActvSub WT-male-NaCl-WT-female-ActvSub 0.9314567
WT-male-ActvSub-WT-female-NaCl WT-male-ActvSub-WT-female-NaCl 0.9999155
WT-male-NaCl-WT-female-NaCl WT-male-NaCl-WT-female-NaCl 0.9769633
WT-male-NaCl-WT-male-ActvSub WT-male-NaCl-WT-male-ActvSub 0.9990665

Additional analysis for parameter: Latency to fall coefficient [s]

Statistical Decision Pipeline:
1. Data recognized as right-censored (300s max threshold).
2. Selected pathway: KAPLAN-MEIER & COX PROPORTIONAL HAZARDS MODEL.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw latency data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 121.080 123.542 43.571 33.160
Group 2 21 115.218 112.133 58.974 50.265
Sex female 23 118.931 122.320 48.065 35.086
Sex male 20 117.397 104.288 55.699 37.872
Genotype KO 18 102.863 103.773 46.790 40.350
Genotype WT 25 129.273 122.320 52.153 33.213
Factor ActvSub 22 121.535 115.402 44.326 36.535
Factor NaCl 21 114.742 104.380 58.326 46.758
Interaction KO-female-ActvSub 5 137.847 161.820 57.072 7.448
Interaction KO-female-NaCl 5 101.062 103.350 51.760 39.912
Interaction KO-male-ActvSub 4 93.813 92.915 16.541 12.656
Interaction KO-male-NaCl 4 70.432 65.072 25.700 15.223
Interaction WT-female-ActvSub 7 116.144 116.107 48.952 16.781
Interaction WT-female-NaCl 6 121.311 123.288 42.960 26.271
Interaction WT-male-ActvSub 6 132.713 136.923 39.181 32.421
Interaction WT-male-NaCl 6 149.112 143.543 77.430 46.987

1. Main pairwise comparisons (Kaplan-Meier estimates)

Summary of main comparisons (Log-Rank Test)
Comparison P-value (Log-rank)
Group: 1 vs 2 0.7783571
Sex: male vs female 0.9707138
Genotype: WT vs KO 0.0975228
Factor: NaCl vs ActvSub 0.8859056

2. Sex-Genotype-Factor Interaction (Survival Curves)

3-Way Interaction Assessment (Cox Proportional Hazards Model)
Term P-value
GenotypeWT 0.4471
Sexmale 0.0294
ModNaCl 0.1354
GenotypeWT:Sexmale 0.0587
GenotypeWT:ModNaCl 0.2702
Sexmale:ModNaCl 0.6256
GenotypeWT:Sexmale:ModNaCl 0.8455
Pairwise comparisons for interaction (Pairwise Log-Rank)
P-values adjusted with Holm’s method.
Comparison P-value (Holm)
WT-male-ActvSub vs KO-male-NaCl 0.4315400
WT-female-ActvSub vs KO-male-NaCl 0.7467133
WT-female-NaCl vs KO-male-NaCl 0.7467133
KO-female-NaCl vs KO-female-ActvSub 1.0000000
KO-male-ActvSub vs KO-female-ActvSub 1.0000000
KO-male-NaCl vs KO-female-ActvSub 1.0000000
WT-female-ActvSub vs KO-female-ActvSub 1.0000000
WT-female-NaCl vs KO-female-ActvSub 1.0000000
WT-male-ActvSub vs KO-female-ActvSub 1.0000000
WT-male-NaCl vs KO-female-ActvSub 1.0000000
KO-male-ActvSub vs KO-female-NaCl 1.0000000
KO-male-NaCl vs KO-female-NaCl 1.0000000
WT-female-ActvSub vs KO-female-NaCl 1.0000000
WT-female-NaCl vs KO-female-NaCl 1.0000000
WT-male-ActvSub vs KO-female-NaCl 1.0000000
WT-male-NaCl vs KO-female-NaCl 1.0000000
KO-male-NaCl vs KO-male-ActvSub 1.0000000
WT-female-ActvSub vs KO-male-ActvSub 1.0000000
WT-female-NaCl vs KO-male-ActvSub 1.0000000
WT-male-ActvSub vs KO-male-ActvSub 1.0000000
WT-male-NaCl vs KO-male-ActvSub 1.0000000
WT-male-NaCl vs KO-male-NaCl 1.0000000
WT-female-NaCl vs WT-female-ActvSub 1.0000000
WT-male-ActvSub vs WT-female-ActvSub 1.0000000
WT-male-NaCl vs WT-female-ActvSub 1.0000000
WT-male-ActvSub vs WT-female-NaCl 1.0000000
WT-male-NaCl vs WT-female-NaCl 1.0000000
WT-male-NaCl vs WT-male-ActvSub 1.0000000

Grip-test Results:

Analysis for parameter: Peak force coefficient [N]

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Raw data.
3. Selected pathway: PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 2.520 2.497 0.328 0.292
Group 2 21 2.575 2.640 0.319 0.245
Sex female 23 2.370 2.350 0.276 0.190
Sex male 20 2.750 2.818 0.241 0.155
Genotype KO 18 2.544 2.517 0.325 0.280
Genotype WT 25 2.549 2.523 0.324 0.247
Factor ActvSub 22 2.553 2.573 0.302 0.238
Factor NaCl 21 2.541 2.480 0.347 0.347
Interaction KO-female-ActvSub 5 2.355 2.253 0.324 0.202
Interaction KO-female-NaCl 5 2.346 2.350 0.207 0.152
Interaction KO-male-ActvSub 4 2.872 2.865 0.126 0.064
Interaction KO-male-NaCl 4 2.698 2.680 0.293 0.224
Interaction WT-female-ActvSub 7 2.440 2.513 0.232 0.086
Interaction WT-female-NaCl 6 2.322 2.157 0.376 0.126
Interaction WT-male-ActvSub 6 2.636 2.737 0.280 0.177
Interaction WT-male-NaCl 6 2.817 2.920 0.217 0.070

1. Main comparisons

Summary of main comparisons

Test used: Welch Two Sample t-test

Comparison P-value
Group: 1 vs 2 0.5753638
Sex: male vs female 0.0000200
Genotype: WT vs KO 0.9587510
Factor: NaCl vs ActvSub 0.9049621

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: 3-Way ANOVA (Genotype x Sex x Mod)

Df Sum Sq Mean Sq F value Pr(>F)
Genotype 1 0.0003 0.0003 0.0039 0.9504
Sex 1 1.5452 1.5452 21.1949 0.0001
Mod 1 0.0044 0.0044 0.0610 0.8064
Genotype:Sex 1 0.0225 0.0225 0.3083 0.5822
Genotype:Mod 1 0.0305 0.0305 0.4184 0.5220
Sex:Mod 1 0.0295 0.0295 0.4042 0.5291
Genotype:Sex:Mod 1 0.1395 0.1395 1.9134 0.1753
Residuals 35 2.5516 0.0729 NA NA
Pairwise comparisons for interaction

Post-hoc used: Tukey HSD

Comparison P-value (Tukey)
KO-female-NaCl-KO-female-ActvSub KO-female-NaCl-KO-female-ActvSub 1.0000000
KO-male-ActvSub-KO-female-ActvSub KO-male-ActvSub-KO-female-ActvSub 0.1128085
KO-male-NaCl-KO-female-ActvSub KO-male-NaCl-KO-female-ActvSub 0.5616507
WT-female-ActvSub-KO-female-ActvSub WT-female-ActvSub-KO-female-ActvSub 0.9993467
WT-female-NaCl-KO-female-ActvSub WT-female-NaCl-KO-female-ActvSub 0.9999991
WT-male-ActvSub-KO-female-ActvSub WT-male-ActvSub-KO-female-ActvSub 0.6738780
WT-male-NaCl-KO-female-ActvSub WT-male-NaCl-KO-female-ActvSub 0.1209858
KO-male-ActvSub-KO-female-NaCl KO-male-ActvSub-KO-female-NaCl 0.1018429
KO-male-NaCl-KO-female-NaCl KO-male-NaCl-KO-female-NaCl 0.5309862
WT-female-ActvSub-KO-female-NaCl WT-female-ActvSub-KO-female-NaCl 0.9987749
WT-female-NaCl-KO-female-NaCl WT-female-NaCl-KO-female-NaCl 0.9999999
WT-male-ActvSub-KO-female-NaCl WT-male-ActvSub-KO-female-NaCl 0.6404938
WT-male-NaCl-KO-female-NaCl WT-male-NaCl-KO-female-NaCl 0.1081626
KO-male-NaCl-KO-male-ActvSub KO-male-NaCl-KO-male-ActvSub 0.9828457
WT-female-ActvSub-KO-male-ActvSub WT-female-ActvSub-KO-male-ActvSub 0.2054835
WT-female-NaCl-KO-male-ActvSub WT-female-NaCl-KO-male-ActvSub 0.0576236
WT-male-ActvSub-KO-male-ActvSub WT-male-ActvSub-KO-male-ActvSub 0.8702531
WT-male-NaCl-KO-male-ActvSub WT-male-NaCl-KO-male-ActvSub 0.9999793
WT-female-ActvSub-KO-male-NaCl WT-female-ActvSub-KO-male-NaCl 0.7866624
WT-female-NaCl-KO-male-NaCl WT-female-NaCl-KO-male-NaCl 0.3983844
WT-male-ActvSub-KO-male-NaCl WT-male-ActvSub-KO-male-NaCl 0.9999568
WT-male-NaCl-KO-male-NaCl WT-male-NaCl-KO-male-NaCl 0.9970696
WT-female-NaCl-WT-female-ActvSub WT-female-NaCl-WT-female-ActvSub 0.9928962
WT-male-ActvSub-WT-female-ActvSub WT-male-ActvSub-WT-female-ActvSub 0.8894349
WT-male-NaCl-WT-female-ActvSub WT-male-NaCl-WT-female-ActvSub 0.2243861
WT-male-ActvSub-WT-female-NaCl WT-male-ActvSub-WT-female-NaCl 0.4855088
WT-male-NaCl-WT-female-NaCl WT-male-NaCl-WT-female-NaCl 0.0556558
WT-male-NaCl-WT-male-ActvSub WT-male-NaCl-WT-male-ActvSub 0.9383133

Open-Field Results:

Analysis for parameter: Distance [cm]

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Raw data.
3. Selected pathway: PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 7365.187 6481.195 3338.179 2559.950
Group 2 21 7419.907 8137.140 2300.245 1500.185
Sex female 23 7306.672 6965.380 2781.681 2010.477
Sex male 20 7489.935 7466.990 2984.850 1713.521
Genotype KO 18 6860.156 6366.730 2529.830 949.241
Genotype WT 25 7774.774 8329.290 3043.517 1955.140
Factor ActvSub 22 6904.787 6366.730 3066.193 2438.790
Factor NaCl 21 7902.230 7541.090 2566.567 1728.635
Interaction KO-female-ActvSub 5 5049.522 5384.850 1330.864 725.600
Interaction KO-female-NaCl 5 9047.992 9322.330 2727.038 1703.095
Interaction KO-male-ActvSub 4 6517.520 5810.925 3105.409 1185.987
Interaction KO-male-NaCl 4 6731.290 6996.345 851.882 455.857
Interaction WT-female-ActvSub 7 8897.334 9595.740 2563.246 1238.068
Interaction WT-female-NaCl 6 5880.758 5646.790 2164.804 1311.582
Interaction WT-male-ActvSub 6 6384.382 7213.455 3867.010 2550.939
Interaction WT-male-NaCl 6 9749.528 9364.045 1967.129 1317.257

1. Main comparisons

Summary of main comparisons

Test used: Welch Two Sample t-test

Comparison P-value
Group: 1 vs 2 0.9502355
Sex: male vs female 0.8368845
Genotype: WT vs KO 0.2895283
Factor: NaCl vs ActvSub 0.2533922

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: 3-Way ANOVA (Genotype x Sex x Mod)

Df Sum Sq Mean Sq F value Pr(>F)
Genotype 1 8754340.6 8754340.6 1.3623 0.2510
Sex 1 245677.6 245677.6 0.0382 0.8461
Mod 1 11010651.1 11010651.1 1.7135 0.1991
Genotype:Sex 1 2330061.5 2330061.5 0.3626 0.5509
Genotype:Mod 1 13348922.0 13348922.0 2.0773 0.1584
Sex:Mod 1 12277560.3 12277560.3 1.9106 0.1757
Genotype:Sex:Mod 1 66990096.5 66990096.5 10.4249 0.0027
Residuals 35 224909637.4 6425989.6 NA NA
Pairwise comparisons for interaction

Post-hoc used: Tukey HSD

Comparison P-value (Tukey)
KO-female-NaCl-KO-female-ActvSub KO-female-NaCl-KO-female-ActvSub 0.2312613
KO-male-ActvSub-KO-female-ActvSub KO-male-ActvSub-KO-female-ActvSub 0.9875059
KO-male-NaCl-KO-female-ActvSub KO-male-NaCl-KO-female-ActvSub 0.9730710
WT-female-ActvSub-KO-female-ActvSub WT-female-ActvSub-KO-female-ActvSub 0.1928052
WT-female-NaCl-KO-female-ActvSub WT-female-NaCl-KO-female-ActvSub 0.9993078
WT-male-ActvSub-KO-female-ActvSub WT-male-ActvSub-KO-female-ActvSub 0.9869637
WT-male-NaCl-KO-female-ActvSub WT-male-NaCl-KO-female-ActvSub 0.0722744
KO-male-ActvSub-KO-female-NaCl KO-male-ActvSub-KO-female-NaCl 0.8084111
KO-male-NaCl-KO-female-NaCl KO-male-NaCl-KO-female-NaCl 0.8676892
WT-female-ActvSub-KO-female-NaCl WT-female-ActvSub-KO-female-NaCl 1.0000000
WT-female-NaCl-KO-female-NaCl WT-female-NaCl-KO-female-NaCl 0.4568443
WT-male-ActvSub-KO-female-NaCl WT-male-ActvSub-KO-female-NaCl 0.6652145
WT-male-NaCl-KO-female-NaCl WT-male-NaCl-KO-female-NaCl 0.9997732
KO-male-NaCl-KO-male-ActvSub KO-male-NaCl-KO-male-ActvSub 1.0000000
WT-female-ActvSub-KO-male-ActvSub WT-female-ActvSub-KO-male-ActvSub 0.8033715
WT-female-NaCl-KO-male-ActvSub WT-female-NaCl-KO-male-ActvSub 0.9999227
WT-male-ActvSub-KO-male-ActvSub WT-male-ActvSub-KO-male-ActvSub 1.0000000
WT-male-NaCl-KO-male-ActvSub WT-male-NaCl-KO-male-ActvSub 0.5119309
WT-female-ActvSub-KO-male-NaCl WT-female-ActvSub-KO-male-NaCl 0.8673066
WT-female-NaCl-KO-male-NaCl WT-female-NaCl-KO-male-NaCl 0.9994701
WT-male-ActvSub-KO-male-NaCl WT-male-ActvSub-KO-male-NaCl 0.9999988
WT-male-NaCl-KO-male-NaCl WT-male-NaCl-KO-male-NaCl 0.5956360
WT-female-NaCl-WT-female-ActvSub WT-female-NaCl-WT-female-ActvSub 0.4113778
WT-male-ActvSub-WT-female-ActvSub WT-male-ActvSub-WT-female-ActvSub 0.6357777
WT-male-NaCl-WT-female-ActvSub WT-male-NaCl-WT-female-ActvSub 0.9985964
WT-male-ActvSub-WT-female-NaCl WT-male-ActvSub-WT-female-NaCl 0.9999664
WT-male-NaCl-WT-female-NaCl WT-male-NaCl-WT-female-NaCl 0.1748073
WT-male-NaCl-WT-male-ActvSub WT-male-NaCl-WT-male-ActvSub 0.3224089

Analysis for parameter: Velocity [cm/s]

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Log1p Transformed.
3. Selected pathway: PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 8.587 5.645 7.989 1.695
Group 2 21 6.506 5.240 4.920 0.805
Sex female 23 10.114 5.770 8.256 5.135
Sex male 20 4.646 4.800 1.647 0.983
Genotype KO 18 5.613 4.195 6.110 0.749
Genotype WT 25 8.980 5.870 6.824 1.190
Factor ActvSub 22 6.048 5.190 4.606 1.029
Factor NaCl 21 9.166 5.560 8.127 1.475
Interaction KO-female-ActvSub 5 4.182 4.210 1.269 0.200
Interaction KO-female-NaCl 5 10.098 5.790 10.992 0.660
Interaction KO-male-ActvSub 4 3.473 3.465 0.697 0.326
Interaction KO-male-NaCl 4 3.935 3.875 1.020 0.353
Interaction WT-female-ActvSub 7 9.963 6.560 6.466 3.598
Interaction WT-female-NaCl 6 15.247 15.195 9.218 7.194
Interaction WT-male-ActvSub 6 4.752 5.425 2.257 0.955
Interaction WT-male-NaCl 6 5.797 5.625 1.091 0.354

1. Main comparisons

Summary of main comparisons

Test used: Welch Two Sample t-test

Comparison P-value
Group: 1 vs 2 0.5767100
Sex: male vs female 0.0024232
Genotype: WT vs KO 0.0229034
Factor: NaCl vs ActvSub 0.1002625

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: 3-Way ANOVA (Genotype x Sex x Mod)

Df Sum Sq Mean Sq F value Pr(>F)
Genotype 1 1.6094 1.6094 6.8192 0.0132
Sex 1 2.9517 2.9517 12.5072 0.0012
Mod 1 1.0633 1.0633 4.5054 0.0409
Genotype:Sex 1 0.2776 0.2776 1.1764 0.2855
Genotype:Mod 1 0.0008 0.0008 0.0035 0.9532
Sex:Mod 1 0.1414 0.1414 0.5992 0.4441
Genotype:Sex:Mod 1 0.0728 0.0728 0.3086 0.5821
Residuals 35 8.2600 0.2360 NA NA
Pairwise comparisons for interaction

Post-hoc used: Tukey HSD

Comparison P-value (Tukey)
KO-female-NaCl-KO-female-ActvSub KO-female-NaCl-KO-female-ActvSub 0.6971060
KO-male-ActvSub-KO-female-ActvSub KO-male-ActvSub-KO-female-ActvSub 0.9999142
KO-male-NaCl-KO-female-ActvSub KO-male-NaCl-KO-female-ActvSub 1.0000000
WT-female-ActvSub-KO-female-ActvSub WT-female-ActvSub-KO-female-ActvSub 0.3284130
WT-female-NaCl-KO-female-ActvSub WT-female-NaCl-KO-female-ActvSub 0.0312004
WT-male-ActvSub-KO-female-ActvSub WT-male-ActvSub-KO-female-ActvSub 1.0000000
WT-male-NaCl-KO-female-ActvSub WT-male-NaCl-KO-female-ActvSub 0.9739378
KO-male-ActvSub-KO-female-NaCl KO-male-ActvSub-KO-female-NaCl 0.5071581
KO-male-NaCl-KO-female-NaCl KO-male-NaCl-KO-female-NaCl 0.6859889
WT-female-ActvSub-KO-female-NaCl WT-female-ActvSub-KO-female-NaCl 0.9997307
WT-female-NaCl-KO-female-NaCl WT-female-NaCl-KO-female-NaCl 0.7135733
WT-male-ActvSub-KO-female-NaCl WT-male-ActvSub-KO-female-NaCl 0.7050664
WT-male-NaCl-KO-female-NaCl WT-male-NaCl-KO-female-NaCl 0.9933711
KO-male-NaCl-KO-male-ActvSub KO-male-NaCl-KO-male-ActvSub 0.9999942
WT-female-ActvSub-KO-male-ActvSub WT-female-ActvSub-KO-male-ActvSub 0.2048117
WT-female-NaCl-KO-male-ActvSub WT-female-NaCl-KO-male-ActvSub 0.0188543
WT-male-ActvSub-KO-male-ActvSub WT-male-ActvSub-KO-male-ActvSub 0.9996248
WT-male-NaCl-KO-male-ActvSub WT-male-NaCl-KO-male-ActvSub 0.8798910
WT-female-ActvSub-KO-male-NaCl WT-female-ActvSub-KO-male-NaCl 0.3433339
WT-female-NaCl-KO-male-NaCl WT-female-NaCl-KO-male-NaCl 0.0391584
WT-male-ActvSub-KO-male-NaCl WT-male-ActvSub-KO-male-NaCl 0.9999992
WT-male-NaCl-KO-male-NaCl WT-male-NaCl-KO-male-NaCl 0.9644546
WT-female-NaCl-WT-female-ActvSub WT-female-NaCl-WT-female-ActvSub 0.8891042
WT-male-ActvSub-WT-female-ActvSub WT-male-ActvSub-WT-female-ActvSub 0.3158104
WT-male-NaCl-WT-female-ActvSub WT-male-NaCl-WT-female-ActvSub 0.8779201
WT-male-ActvSub-WT-female-NaCl WT-male-ActvSub-WT-female-NaCl 0.0259040
WT-male-NaCl-WT-female-NaCl WT-male-NaCl-WT-female-NaCl 0.2083281
WT-male-NaCl-WT-male-ActvSub WT-male-NaCl-WT-male-ActvSub 0.9798174

Analysis for parameter: Time [s] spent in the center

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Raw data (Assumptions severely violated).
3. Selected pathway: NON-PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 333.436 258.935 250.600 157.129
Group 2 21 232.309 177.000 167.143 57.535
Sex female 23 342.700 225.310 267.184 203.150
Sex male 20 216.600 189.220 113.969 63.460
Genotype KO 18 225.349 183.590 178.265 62.006
Genotype WT 25 326.312 261.440 236.335 145.435
Factor ActvSub 22 267.117 198.340 194.852 84.889
Factor NaCl 21 301.786 218.410 242.610 109.380
Interaction KO-female-ActvSub 5 171.474 177.000 44.937 34.660
Interaction KO-female-NaCl 5 273.910 116.740 315.842 80.215
Interaction KO-male-ActvSub 4 277.598 224.485 131.772 54.329
Interaction KO-male-NaCl 4 179.743 136.745 108.778 40.794
Interaction WT-female-ActvSub 7 404.731 336.940 261.394 159.982
Interaction WT-female-NaCl 6 470.342 536.120 302.826 251.808
Interaction WT-male-ActvSub 6 179.283 151.435 142.938 16.785
Interaction WT-male-NaCl 6 237.822 239.925 71.651 56.829

1. Main comparisons

Summary of main comparisons

Test used: Wilcoxon Rank Sum Test

Comparison P-value
Group: 1 vs 2 0.1639979
Sex: male vs female 0.4185818
Genotype: WT vs KO 0.1129638
Factor: NaCl vs ActvSub 0.8569048

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: Kruskal-Wallis Rank Sum Test

  • chi-squared = 8.758
  • degrees of freedom (df) = 7
  • p-value = 0.27
Pairwise comparisons for interaction

Post-hoc used: Conover’s test with Holm’s adjustment

Comparison P-value (Holm)
KO-female-ActvSub-KO-female-NaCl 0.9795303
KO-female-ActvSub-KO-male-ActvSub 1.0000000
KO-female-NaCl-KO-male-ActvSub 1.0000000
KO-female-ActvSub-KO-male-NaCl 1.0000000
KO-female-NaCl-KO-male-NaCl 1.0000000
KO-male-ActvSub-KO-male-NaCl 1.0000000
KO-female-ActvSub-WT-female-ActvSub 1.0000000
KO-female-NaCl-WT-female-ActvSub 1.0000000
KO-male-ActvSub-WT-female-ActvSub 1.0000000
KO-male-NaCl-WT-female-ActvSub 0.9852524
KO-female-ActvSub-WT-female-NaCl 1.0000000
KO-female-NaCl-WT-female-NaCl 1.0000000
KO-male-ActvSub-WT-female-NaCl 1.0000000
KO-male-NaCl-WT-female-NaCl 1.0000000
WT-female-ActvSub-WT-female-NaCl 1.0000000
KO-female-ActvSub-WT-male-ActvSub 1.0000000
KO-female-NaCl-WT-male-ActvSub 0.4964363
KO-male-ActvSub-WT-male-ActvSub 1.0000000
KO-male-NaCl-WT-male-ActvSub 1.0000000
WT-female-ActvSub-WT-male-ActvSub 0.8761721
WT-female-NaCl-WT-male-ActvSub 1.0000000
KO-female-ActvSub-WT-male-NaCl 1.0000000
KO-female-NaCl-WT-male-NaCl 1.0000000
KO-male-ActvSub-WT-male-NaCl 1.0000000
KO-male-NaCl-WT-male-NaCl 1.0000000
WT-female-ActvSub-WT-male-NaCl 1.0000000
WT-female-NaCl-WT-male-NaCl 1.0000000
WT-male-ActvSub-WT-male-NaCl 1.0000000

Analysis for parameter: Time [s] spent in the sides

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Raw data (Assumptions severely violated).
3. Selected pathway: NON-PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 1466.564 1541.065 250.600 157.129
Group 2 21 1567.691 1623.000 167.143 57.535
Sex female 23 1457.300 1574.690 267.184 203.150
Sex male 20 1583.400 1610.780 113.969 63.460
Genotype KO 18 1574.651 1616.410 178.265 62.006
Genotype WT 25 1473.688 1538.560 236.335 145.435
Factor ActvSub 22 1532.883 1601.660 194.852 84.889
Factor NaCl 21 1498.214 1581.590 242.610 109.380
Interaction KO-female-ActvSub 5 1628.526 1623.000 44.937 34.660
Interaction KO-female-NaCl 5 1526.090 1683.260 315.842 80.215
Interaction KO-male-ActvSub 4 1522.402 1575.515 131.772 54.329
Interaction KO-male-NaCl 4 1620.257 1663.255 108.778 40.794
Interaction WT-female-ActvSub 7 1395.269 1463.060 261.394 159.983
Interaction WT-female-NaCl 6 1329.658 1263.880 302.826 251.808
Interaction WT-male-ActvSub 6 1620.717 1648.565 142.938 16.785
Interaction WT-male-NaCl 6 1562.178 1560.075 71.651 56.829

1. Main comparisons

Summary of main comparisons

Test used: Wilcoxon Rank Sum Test

Comparison P-value
Group: 1 vs 2 0.1639979
Sex: male vs female 0.4185818
Genotype: WT vs KO 0.1129638
Factor: NaCl vs ActvSub 0.8569048

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: Kruskal-Wallis Rank Sum Test

  • chi-squared = 8.758
  • degrees of freedom (df) = 7
  • p-value = 0.27
Pairwise comparisons for interaction

Post-hoc used: Conover’s test with Holm’s adjustment

Comparison P-value (Holm)
KO-female-ActvSub-KO-female-NaCl 0.9795303
KO-female-ActvSub-KO-male-ActvSub 1.0000000
KO-female-NaCl-KO-male-ActvSub 1.0000000
KO-female-ActvSub-KO-male-NaCl 1.0000000
KO-female-NaCl-KO-male-NaCl 1.0000000
KO-male-ActvSub-KO-male-NaCl 1.0000000
KO-female-ActvSub-WT-female-ActvSub 1.0000000
KO-female-NaCl-WT-female-ActvSub 1.0000000
KO-male-ActvSub-WT-female-ActvSub 1.0000000
KO-male-NaCl-WT-female-ActvSub 0.9852524
KO-female-ActvSub-WT-female-NaCl 1.0000000
KO-female-NaCl-WT-female-NaCl 1.0000000
KO-male-ActvSub-WT-female-NaCl 1.0000000
KO-male-NaCl-WT-female-NaCl 1.0000000
WT-female-ActvSub-WT-female-NaCl 1.0000000
KO-female-ActvSub-WT-male-ActvSub 1.0000000
KO-female-NaCl-WT-male-ActvSub 0.4964363
KO-male-ActvSub-WT-male-ActvSub 1.0000000
KO-male-NaCl-WT-male-ActvSub 1.0000000
WT-female-ActvSub-WT-male-ActvSub 0.8761721
WT-female-NaCl-WT-male-ActvSub 1.0000000
KO-female-ActvSub-WT-male-NaCl 1.0000000
KO-female-NaCl-WT-male-NaCl 1.0000000
KO-male-ActvSub-WT-male-NaCl 1.0000000
KO-male-NaCl-WT-male-NaCl 1.0000000
WT-female-ActvSub-WT-male-NaCl 1.0000000
WT-female-NaCl-WT-male-NaCl 1.0000000
WT-male-ActvSub-WT-male-NaCl 1.0000000

Analysis for parameter: Time [s] spent moving

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Raw data.
3. Selected pathway: PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 1113.134 1158.130 381.629 252.234
Group 2 21 1020.504 960.340 250.958 115.035
Sex female 23 1194.773 1116.320 302.190 221.892
Sex male 20 921.988 934.180 290.661 177.234
Genotype KO 18 917.623 844.825 271.168 140.848
Genotype WT 25 1176.093 1116.320 320.224 175.950
Factor ActvSub 22 1014.407 1016.640 345.629 241.556
Factor NaCl 21 1123.933 1053.810 297.716 210.560
Interaction KO-female-ActvSub 5 896.960 834.320 228.852 69.480
Interaction KO-female-NaCl 5 1153.708 1022.730 333.067 108.405
Interaction KO-male-ActvSub 4 811.908 725.025 204.274 65.556
Interaction KO-male-NaCl 4 754.060 774.765 114.174 73.035
Interaction WT-female-ActvSub 7 1291.729 1241.900 262.956 205.783
Interaction WT-female-NaCl 6 1364.057 1446.770 224.175 165.505
Interaction WT-male-ActvSub 6 923.737 960.710 433.227 156.158
Interaction WT-male-NaCl 6 1105.578 1055.310 166.703 107.486

1. Main comparisons

Summary of main comparisons

Test used: Welch Two Sample t-test

Comparison P-value
Group: 1 vs 2 0.3511480
Sex: male vs female 0.0044376
Genotype: WT vs KO 0.0067726
Factor: NaCl vs ActvSub 0.2714491

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: 3-Way ANOVA (Genotype x Sex x Mod)

Df Sum Sq Mean Sq F value Pr(>F)
Genotype 1 699142.5959 699142.5959 9.5547 0.0039
Sex 1 850418.5158 850418.5158 11.6221 0.0017
Mod 1 157090.0911 157090.0911 2.1468 0.1518
Genotype:Sex 1 13744.8545 13744.8545 0.1878 0.6674
Genotype:Mod 1 172.6655 172.6655 0.0024 0.9615
Sex:Mod 1 12041.7173 12041.7173 0.1646 0.6875
Genotype:Sex:Mod 1 116581.4381 116581.4381 1.5932 0.2152
Residuals 35 2561042.8872 73172.6539 NA NA
Pairwise comparisons for interaction

Post-hoc used: Tukey HSD

Comparison P-value (Tukey)
KO-female-NaCl-KO-female-ActvSub KO-female-NaCl-KO-female-ActvSub 0.8018454
KO-male-ActvSub-KO-female-ActvSub KO-male-ActvSub-KO-female-ActvSub 0.9997319
KO-male-NaCl-KO-female-ActvSub KO-male-NaCl-KO-female-ActvSub 0.9927359
WT-female-ActvSub-KO-female-ActvSub WT-female-ActvSub-KO-female-ActvSub 0.2319378
WT-female-NaCl-KO-female-ActvSub WT-female-NaCl-KO-female-ActvSub 0.1145703
WT-male-ActvSub-KO-female-ActvSub WT-male-ActvSub-KO-female-ActvSub 0.9999998
WT-male-NaCl-KO-female-ActvSub WT-male-NaCl-KO-female-ActvSub 0.9024201
KO-male-ActvSub-KO-female-NaCl KO-male-ActvSub-KO-female-NaCl 0.5705103
KO-male-NaCl-KO-female-NaCl KO-male-NaCl-KO-female-NaCl 0.3748208
WT-female-ActvSub-KO-female-NaCl WT-female-ActvSub-KO-female-NaCl 0.9868094
WT-female-NaCl-KO-female-NaCl WT-female-NaCl-KO-female-NaCl 0.8986095
WT-male-ActvSub-KO-female-NaCl WT-male-ActvSub-KO-female-NaCl 0.8493166
WT-male-NaCl-KO-female-NaCl WT-male-NaCl-KO-female-NaCl 0.9999886
KO-male-NaCl-KO-male-ActvSub KO-male-NaCl-KO-male-ActvSub 0.9999861
WT-female-ActvSub-KO-male-ActvSub WT-female-ActvSub-KO-male-ActvSub 0.1199106
WT-female-NaCl-KO-male-ActvSub WT-female-NaCl-KO-male-ActvSub 0.0573943
WT-male-ActvSub-KO-male-ActvSub WT-male-ActvSub-KO-male-ActvSub 0.9979688
WT-male-NaCl-KO-male-ActvSub WT-male-NaCl-KO-male-ActvSub 0.6983061
WT-female-ActvSub-KO-male-NaCl WT-female-ActvSub-KO-male-NaCl 0.0562002
WT-female-NaCl-KO-male-NaCl WT-female-NaCl-KO-male-NaCl 0.0256724
WT-male-ActvSub-KO-male-NaCl WT-male-ActvSub-KO-male-NaCl 0.9755539
WT-male-NaCl-KO-male-NaCl WT-male-NaCl-KO-male-NaCl 0.4879847
WT-female-NaCl-WT-female-ActvSub WT-female-NaCl-WT-female-ActvSub 0.9996835
WT-male-ActvSub-WT-female-ActvSub WT-male-ActvSub-WT-female-ActvSub 0.2522317
WT-male-NaCl-WT-female-ActvSub WT-male-NaCl-WT-female-ActvSub 0.9149738
WT-male-ActvSub-WT-female-NaCl WT-male-ActvSub-WT-female-NaCl 0.1226048
WT-male-NaCl-WT-female-NaCl WT-male-NaCl-WT-female-NaCl 0.7145824
WT-male-NaCl-WT-male-ActvSub WT-male-NaCl-WT-male-ActvSub 0.9366755

Analysis for parameter: Time [s] spent in the immobility state

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Raw data.
3. Selected pathway: PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 756.417 703.900 396.514 276.721
Group 2 21 839.182 860.550 244.207 130.825
Sex female 23 677.066 774.160 312.312 243.145
Sex male 20 934.574 907.125 300.246 160.456
Genotype KO 18 958.263 1057.835 274.782 138.481
Genotype WT 25 680.610 774.160 321.304 180.685
Factor ActvSub 22 843.364 837.755 344.166 229.985
Factor NaCl 21 748.094 801.870 314.886 201.515
Interaction KO-female-ActvSub 5 973.068 1046.160 240.182 141.120
Interaction KO-female-NaCl 5 746.794 860.550 351.052 103.540
Interaction KO-male-ActvSub 4 1015.040 1099.690 219.533 67.510
Interaction KO-male-NaCl 4 1147.315 1141.435 107.641 79.318
Interaction WT-female-ActvSub 7 583.366 649.040 255.871 195.525
Interaction WT-female-NaCl 6 481.607 377.300 235.058 158.855
Interaction WT-male-ActvSub 6 924.158 880.425 448.316 136.720
Interaction WT-male-NaCl 6 749.518 811.450 151.965 98.466

1. Main comparisons

Summary of main comparisons

Test used: Welch Two Sample t-test

Comparison P-value
Group: 1 vs 2 0.4131334
Sex: male vs female 0.0087889
Genotype: WT vs KO 0.0041394
Factor: NaCl vs ActvSub 0.3488422

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: 3-Way ANOVA (Genotype x Sex x Mod)

Df Sum Sq Mean Sq F value Pr(>F)
Genotype 1 806764.63 806764.63 10.4871 0.0026
Sex 1 764519.57 764519.57 9.9379 0.0033
Mod 1 122324.66 122324.66 1.5901 0.2157
Genotype:Sex 1 18016.49 18016.49 0.2342 0.6314
Genotype:Mod 1 12777.99 12777.99 0.1661 0.6861
Sex:Mod 1 30463.10 30463.10 0.3960 0.5333
Genotype:Sex:Mod 1 120640.98 120640.98 1.5682 0.2188
Residuals 35 2692525.94 76929.31 NA NA
Pairwise comparisons for interaction

Post-hoc used: Tukey HSD

Comparison P-value (Tukey)
KO-female-NaCl-KO-female-ActvSub KO-female-NaCl-KO-female-ActvSub 0.8965105
KO-male-ActvSub-KO-female-ActvSub KO-male-ActvSub-KO-female-ActvSub 0.9999981
KO-male-NaCl-KO-female-ActvSub KO-male-NaCl-KO-female-ActvSub 0.9801010
WT-female-ActvSub-KO-female-ActvSub WT-female-ActvSub-KO-female-ActvSub 0.2730049
WT-female-NaCl-KO-female-ActvSub WT-female-NaCl-KO-female-ActvSub 0.0976589
WT-male-ActvSub-KO-female-ActvSub WT-male-ActvSub-KO-female-ActvSub 0.9999892
WT-male-NaCl-KO-female-ActvSub WT-male-NaCl-KO-female-ActvSub 0.8806492
KO-male-ActvSub-KO-female-NaCl KO-male-ActvSub-KO-female-NaCl 0.8315666
KO-male-NaCl-KO-female-NaCl KO-male-NaCl-KO-female-NaCl 0.4033425
WT-female-ActvSub-KO-female-NaCl WT-female-ActvSub-KO-female-NaCl 0.9703983
WT-female-NaCl-KO-female-NaCl WT-female-NaCl-KO-female-NaCl 0.7591346
WT-male-ActvSub-KO-female-NaCl WT-male-ActvSub-KO-female-NaCl 0.9616558
WT-male-NaCl-KO-female-NaCl WT-male-NaCl-KO-female-NaCl 1.0000000
KO-male-NaCl-KO-male-ActvSub KO-male-NaCl-KO-male-ActvSub 0.9971889
WT-female-ActvSub-KO-male-ActvSub WT-female-ActvSub-KO-male-ActvSub 0.2358353
WT-female-NaCl-KO-male-ActvSub WT-female-NaCl-KO-male-ActvSub 0.0869129
WT-male-ActvSub-KO-male-ActvSub WT-male-ActvSub-KO-male-ActvSub 0.9995463
WT-male-NaCl-KO-male-ActvSub WT-male-NaCl-KO-male-ActvSub 0.8109841
WT-female-ActvSub-KO-male-NaCl WT-female-ActvSub-KO-male-NaCl 0.0473330
WT-female-NaCl-KO-male-NaCl WT-female-NaCl-KO-male-NaCl 0.0144019
WT-male-ActvSub-KO-male-NaCl WT-male-ActvSub-KO-male-NaCl 0.9118226
WT-male-NaCl-KO-male-NaCl WT-male-NaCl-KO-male-NaCl 0.3639401
WT-female-NaCl-WT-female-ActvSub WT-female-NaCl-WT-female-ActvSub 0.9975582
WT-male-ActvSub-WT-female-ActvSub WT-male-ActvSub-WT-female-ActvSub 0.3713980
WT-male-NaCl-WT-female-ActvSub WT-male-NaCl-WT-female-ActvSub 0.9575322
WT-male-ActvSub-WT-female-NaCl WT-male-ActvSub-WT-female-NaCl 0.1375725
WT-male-NaCl-WT-female-NaCl WT-male-NaCl-WT-female-NaCl 0.7036921
WT-male-NaCl-WT-male-ActvSub WT-male-NaCl-WT-male-ActvSub 0.9546097

Analysis for parameter: Number of clockwise rotations

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Square Root Transformed (Count Data Fix).
3. Selected pathway: PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 45.773 20.5 59.942 18.875
Group 2 21 40.667 25.0 43.729 18.500
Sex female 23 59.652 36.0 64.008 23.250
Sex male 20 24.450 21.0 23.607 12.500
Genotype KO 18 36.833 23.5 44.234 13.625
Genotype WT 25 47.920 24.0 57.529 26.000
Factor ActvSub 22 31.318 20.0 36.324 19.500
Factor NaCl 21 55.810 31.0 63.176 20.500
Interaction KO-female-ActvSub 5 37.600 47.0 16.149 14.500
Interaction KO-female-NaCl 5 69.000 46.0 75.541 18.000
Interaction KO-male-ActvSub 4 16.000 21.0 10.677 2.750
Interaction KO-male-NaCl 4 16.500 19.5 12.715 8.250
Interaction WT-female-ActvSub 7 35.714 11.0 56.588 20.750
Interaction WT-female-NaCl 6 98.167 97.5 79.083 67.500
Interaction WT-male-ActvSub 6 31.167 15.0 34.173 19.125
Interaction WT-male-NaCl 6 28.667 34.0 24.525 16.500

1. Main comparisons

Summary of main comparisons

Test used: Welch Two Sample t-test

Comparison P-value
Group: 1 vs 2 0.9063160
Sex: male vs female 0.0255923
Genotype: WT vs KO 0.8031829
Factor: NaCl vs ActvSub 0.1474240

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: 3-Way ANOVA (Genotype x Sex x Mod)

Df Sum Sq Mean Sq F value Pr(>F)
Genotype 1 3.0958 3.0958 0.0650 0.8003
Sex 1 250.7372 250.7372 5.2625 0.0279
Mod 1 123.7185 123.7185 2.5966 0.1161
Genotype:Sex 1 14.1474 14.1474 0.2969 0.5893
Genotype:Mod 1 23.3607 23.3607 0.4903 0.4884
Sex:Mod 1 131.1438 131.1438 2.7524 0.1060
Genotype:Sex:Mod 1 32.1784 32.1784 0.6754 0.4168
Residuals 35 1667.6228 47.6464 NA NA
Pairwise comparisons for interaction

Post-hoc used: Tukey HSD

Comparison P-value (Tukey)
KO-female-NaCl-KO-female-ActvSub KO-female-NaCl-KO-female-ActvSub 0.9972908
KO-male-ActvSub-KO-female-ActvSub KO-male-ActvSub-KO-female-ActvSub 0.9639873
KO-male-NaCl-KO-female-ActvSub KO-male-NaCl-KO-female-ActvSub 0.9649487
WT-female-ActvSub-KO-female-ActvSub WT-female-ActvSub-KO-female-ActvSub 0.9910131
WT-female-NaCl-KO-female-ActvSub WT-female-NaCl-KO-female-ActvSub 0.8206943
WT-male-ActvSub-KO-female-ActvSub WT-male-ActvSub-KO-female-ActvSub 0.9986663
WT-male-NaCl-KO-female-ActvSub WT-male-NaCl-KO-female-ActvSub 0.9955414
KO-male-ActvSub-KO-female-NaCl KO-male-ActvSub-KO-female-NaCl 0.7020268
KO-male-NaCl-KO-female-NaCl KO-male-NaCl-KO-female-NaCl 0.7052404
WT-female-ActvSub-KO-female-NaCl WT-female-ActvSub-KO-female-NaCl 0.7806637
WT-female-NaCl-KO-female-NaCl WT-female-NaCl-KO-female-NaCl 0.9939684
WT-male-ActvSub-KO-female-NaCl WT-male-ActvSub-KO-female-NaCl 0.8928405
WT-male-NaCl-KO-female-NaCl WT-male-NaCl-KO-female-NaCl 0.8386616
KO-male-NaCl-KO-male-ActvSub KO-male-NaCl-KO-male-ActvSub 1.0000000
WT-female-ActvSub-KO-male-ActvSub WT-female-ActvSub-KO-male-ActvSub 0.9999588
WT-female-NaCl-KO-male-ActvSub WT-female-NaCl-KO-male-ActvSub 0.2467003
WT-male-ActvSub-KO-male-ActvSub WT-male-ActvSub-KO-male-ActvSub 0.9994535
WT-male-NaCl-KO-male-ActvSub WT-male-NaCl-KO-male-ActvSub 0.9999022
WT-female-ActvSub-KO-male-NaCl WT-female-ActvSub-KO-male-NaCl 0.9999631
WT-female-NaCl-KO-male-NaCl WT-female-NaCl-KO-male-NaCl 0.2491022
WT-male-ActvSub-KO-male-NaCl WT-male-ActvSub-KO-male-NaCl 0.9994900
WT-male-NaCl-KO-male-NaCl WT-male-NaCl-KO-male-NaCl 0.9999108
WT-female-NaCl-WT-female-ActvSub WT-female-NaCl-WT-female-ActvSub 0.2491476
WT-male-ActvSub-WT-female-ActvSub WT-male-ActvSub-WT-female-ActvSub 0.9999990
WT-male-NaCl-WT-female-ActvSub WT-male-NaCl-WT-female-ActvSub 1.0000000
WT-male-ActvSub-WT-female-NaCl WT-male-ActvSub-WT-female-NaCl 0.3967438
WT-male-NaCl-WT-female-NaCl WT-male-NaCl-WT-female-NaCl 0.3234079
WT-male-NaCl-WT-male-ActvSub WT-male-NaCl-WT-male-ActvSub 1.0000000

Analysis for parameter: Number of counter-clockwise rotations

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Raw data (Assumptions severely violated).
3. Selected pathway: NON-PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 34.136 18.5 53.235 10.125
Group 2 21 26.905 25.0 15.159 12.500
Sex female 23 36.217 18.0 51.480 10.500
Sex male 20 24.150 25.0 15.909 11.625
Genotype KO 18 21.111 23.0 12.704 7.125
Genotype WT 25 37.440 26.0 49.639 13.500
Factor ActvSub 22 30.773 21.5 40.593 10.875
Factor NaCl 21 30.429 25.0 38.772 10.500
Interaction KO-female-ActvSub 5 22.000 18.0 17.029 11.000
Interaction KO-female-NaCl 5 24.800 25.0 10.281 1.000
Interaction KO-male-ActvSub 4 25.500 23.0 6.455 2.500
Interaction KO-male-NaCl 4 11.000 8.0 12.623 7.250
Interaction WT-female-ActvSub 7 47.429 17.0 69.510 14.000
Interaction WT-female-NaCl 6 44.500 14.5 70.395 8.000
Interaction WT-male-ActvSub 6 22.167 20.5 17.011 11.500
Interaction WT-male-NaCl 6 34.000 36.0 17.053 5.125

1. Main comparisons

Summary of main comparisons

Test used: Wilcoxon Rank Sum Test

Comparison P-value
Group: 1 vs 2 0.2686581
Sex: male vs female 0.9320437
Genotype: WT vs KO 0.3954114
Factor: NaCl vs ActvSub 0.9515361

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: Kruskal-Wallis Rank Sum Test

  • chi-squared = 5.669
  • degrees of freedom (df) = 7
  • p-value = 0.579
Pairwise comparisons for interaction

Post-hoc used: Conover’s test with Holm’s adjustment

Comparison P-value (Holm)
KO-female-ActvSub-KO-female-NaCl 1.0000000
KO-female-ActvSub-KO-male-ActvSub 1.0000000
KO-female-NaCl-KO-male-ActvSub 1.0000000
KO-female-ActvSub-KO-male-NaCl 1.0000000
KO-female-NaCl-KO-male-NaCl 1.0000000
KO-male-ActvSub-KO-male-NaCl 1.0000000
KO-female-ActvSub-WT-female-ActvSub 1.0000000
KO-female-NaCl-WT-female-ActvSub 0.4909305
KO-male-ActvSub-WT-female-ActvSub 1.0000000
KO-male-NaCl-WT-female-ActvSub 1.0000000
KO-female-ActvSub-WT-female-NaCl 1.0000000
KO-female-NaCl-WT-female-NaCl 1.0000000
KO-male-ActvSub-WT-female-NaCl 1.0000000
KO-male-NaCl-WT-female-NaCl 1.0000000
WT-female-ActvSub-WT-female-NaCl 1.0000000
KO-female-ActvSub-WT-male-ActvSub 0.9812419
KO-female-NaCl-WT-male-ActvSub 1.0000000
KO-male-ActvSub-WT-male-ActvSub 1.0000000
KO-male-NaCl-WT-male-ActvSub 1.0000000
WT-female-ActvSub-WT-male-ActvSub 1.0000000
WT-female-NaCl-WT-male-ActvSub 1.0000000
KO-female-ActvSub-WT-male-NaCl 1.0000000
KO-female-NaCl-WT-male-NaCl 1.0000000
KO-male-ActvSub-WT-male-NaCl 1.0000000
KO-male-NaCl-WT-male-NaCl 0.4251708
WT-female-ActvSub-WT-male-NaCl 1.0000000
WT-female-NaCl-WT-male-NaCl 1.0000000
WT-male-ActvSub-WT-male-NaCl 1.0000000

Analysis for parameter: Total number of rotations

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Raw data.
3. Selected pathway: PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 79.909 51.5 70.009 34.125
Group 2 21 67.571 55.0 41.137 16.500
Sex female 23 95.870 68.0 68.462 59.250
Sex male 20 48.600 46.0 24.483 16.125
Genotype KO 18 57.944 50.5 42.907 17.250
Genotype WT 25 85.360 57.0 64.305 37.000
Factor ActvSub 22 62.091 51.5 48.626 21.250
Factor NaCl 21 86.238 57.0 64.221 24.000
Interaction KO-female-ActvSub 5 59.600 57.0 20.756 8.500
Interaction KO-female-NaCl 5 93.800 72.0 66.560 16.500
Interaction KO-male-ActvSub 4 41.500 44.0 14.271 5.750
Interaction KO-male-NaCl 4 27.500 27.0 1.915 1.250
Interaction WT-female-ActvSub 7 83.143 48.0 78.766 60.750
Interaction WT-female-NaCl 6 142.667 171.0 71.517 54.875
Interaction WT-male-ActvSub 6 53.333 53.5 31.001 17.250
Interaction WT-male-NaCl 6 62.667 63.0 23.054 14.375

1. Main comparisons

Summary of main comparisons

Test used: Welch Two Sample t-test

Comparison P-value
Group: 1 vs 2 0.4835223
Sex: male vs female 0.0044459
Genotype: WT vs KO 0.1014491
Factor: NaCl vs ActvSub 0.1742242

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: 3-Way ANOVA (Genotype x Sex x Mod)

Df Sum Sq Mean Sq F value Pr(>F)
Genotype 1 7865.7142 7865.7142 3.0478 0.0896
Sex 1 24907.9482 24907.9482 9.6514 0.0037
Mod 1 7135.8410 7135.8410 2.7650 0.1053
Genotype:Sex 1 337.8708 337.8708 0.1309 0.7197
Genotype:Mod 1 1332.2346 1332.2346 0.5162 0.4772
Sex:Mod 1 6497.3848 6497.3848 2.5176 0.1216
Genotype:Sex:Mod 1 2.5680 2.5680 0.0010 0.9750
Residuals 35 90326.8571 2580.7673 NA NA
Pairwise comparisons for interaction

Post-hoc used: Tukey HSD

Comparison P-value (Tukey)
KO-female-NaCl-KO-female-ActvSub KO-female-NaCl-KO-female-ActvSub 0.9600197
KO-male-ActvSub-KO-female-ActvSub KO-male-ActvSub-KO-female-ActvSub 0.9993899
KO-male-NaCl-KO-female-ActvSub KO-male-NaCl-KO-female-ActvSub 0.9794447
WT-female-ActvSub-KO-female-ActvSub WT-female-ActvSub-KO-female-ActvSub 0.9925146
WT-female-NaCl-KO-female-ActvSub WT-female-NaCl-KO-female-ActvSub 0.1563027
WT-male-ActvSub-KO-female-ActvSub WT-male-ActvSub-KO-female-ActvSub 0.9999991
WT-male-NaCl-KO-female-ActvSub WT-male-NaCl-KO-female-ActvSub 1.0000000
KO-male-ActvSub-KO-female-NaCl KO-male-ActvSub-KO-female-NaCl 0.7837359
KO-male-NaCl-KO-female-NaCl KO-male-NaCl-KO-female-NaCl 0.5308187
WT-female-ActvSub-KO-female-NaCl WT-female-ActvSub-KO-female-NaCl 0.9999558
WT-female-NaCl-KO-female-NaCl WT-female-NaCl-KO-female-NaCl 0.7536645
WT-male-ActvSub-KO-female-NaCl WT-male-ActvSub-KO-female-NaCl 0.8868028
WT-male-NaCl-KO-female-NaCl WT-male-NaCl-KO-female-NaCl 0.9694632
KO-male-NaCl-KO-male-ActvSub KO-male-NaCl-KO-male-ActvSub 0.9999219
WT-female-ActvSub-KO-male-ActvSub WT-female-ActvSub-KO-male-ActvSub 0.8897662
WT-female-NaCl-KO-male-ActvSub WT-female-NaCl-KO-male-ActvSub 0.0685635
WT-male-ActvSub-KO-male-ActvSub WT-male-ActvSub-KO-male-ActvSub 0.9999536
WT-male-NaCl-KO-male-ActvSub WT-male-NaCl-KO-male-ActvSub 0.9978659
WT-female-ActvSub-KO-male-NaCl WT-female-ActvSub-KO-male-NaCl 0.6575211
WT-female-NaCl-KO-male-NaCl WT-female-NaCl-KO-male-NaCl 0.0244990
WT-male-ActvSub-KO-male-NaCl WT-male-ActvSub-KO-male-NaCl 0.9927199
WT-male-NaCl-KO-male-NaCl WT-male-NaCl-KO-male-NaCl 0.9584203
WT-female-NaCl-WT-female-ActvSub WT-female-NaCl-WT-female-ActvSub 0.4309229
WT-male-ActvSub-WT-female-ActvSub WT-male-ActvSub-WT-female-ActvSub 0.9619125
WT-male-NaCl-WT-female-ActvSub WT-male-NaCl-WT-female-ActvSub 0.9956209
WT-male-ActvSub-WT-female-NaCl WT-male-ActvSub-WT-female-NaCl 0.0749569
WT-male-NaCl-WT-female-NaCl WT-male-NaCl-WT-female-NaCl 0.1480077
WT-male-NaCl-WT-male-ActvSub WT-male-NaCl-WT-male-ActvSub 0.9999803

Analysis for parameter: Number of clockwise spins

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Square Root Transformed (Count Data Fix).
3. Selected pathway: PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 96.045 93.0 73.014 65.375
Group 2 21 89.048 60.0 95.257 27.000
Sex female 23 109.652 88.0 98.360 51.250
Sex male 20 73.050 59.5 59.285 36.250
Genotype KO 18 75.889 60.0 64.876 40.875
Genotype WT 25 104.680 88.0 94.368 53.500
Factor ActvSub 22 95.091 75.0 88.563 24.500
Factor NaCl 21 90.048 50.0 80.303 65.000
Interaction KO-female-ActvSub 5 71.800 75.0 36.881 14.000
Interaction KO-female-NaCl 5 99.400 50.0 94.551 63.500
Interaction KO-male-ActvSub 4 39.750 41.0 30.772 22.375
Interaction KO-male-NaCl 4 87.750 81.5 79.324 49.125
Interaction WT-female-ActvSub 7 146.714 103.0 130.756 37.750
Interaction WT-female-NaCl 6 106.500 69.0 101.683 74.750
Interaction WT-male-ActvSub 6 91.167 82.5 63.458 35.500
Interaction WT-male-NaCl 6 67.333 49.0 59.095 26.875

1. Main comparisons

Summary of main comparisons

Test used: Welch Two Sample t-test

Comparison P-value
Group: 1 vs 2 0.7604438
Sex: male vs female 0.1646665
Genotype: WT vs KO 0.3091461
Factor: NaCl vs ActvSub 0.6919799

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: 3-Way ANOVA (Genotype x Sex x Mod)

Df Sum Sq Mean Sq F value Pr(>F)
Genotype 1 78.1208 78.1208 0.9519 0.3359
Sex 1 156.3464 156.3464 1.9050 0.1763
Mod 1 9.6141 9.6141 0.1171 0.7342
Genotype:Sex 1 11.7856 11.7856 0.1436 0.7070
Genotype:Mod 1 95.3863 95.3863 1.1622 0.2884
Sex:Mod 1 17.9784 17.9784 0.2191 0.6427
Genotype:Sex:Mod 1 4.9258 4.9258 0.0600 0.8079
Residuals 35 2872.5223 82.0721 NA NA
Pairwise comparisons for interaction

Post-hoc used: Tukey HSD

Comparison P-value (Tukey)
KO-female-NaCl-KO-female-ActvSub KO-female-NaCl-KO-female-ActvSub 1.0000000
KO-male-ActvSub-KO-female-ActvSub KO-male-ActvSub-KO-female-ActvSub 0.9937947
KO-male-NaCl-KO-female-ActvSub KO-male-NaCl-KO-female-ActvSub 1.0000000
WT-female-ActvSub-KO-female-ActvSub WT-female-ActvSub-KO-female-ActvSub 0.9385849
WT-female-NaCl-KO-female-ActvSub WT-female-NaCl-KO-female-ActvSub 0.9999533
WT-male-ActvSub-KO-female-ActvSub WT-male-ActvSub-KO-female-ActvSub 0.9999999
WT-male-NaCl-KO-female-ActvSub WT-male-NaCl-KO-female-ActvSub 0.9999694
KO-male-ActvSub-KO-female-NaCl KO-male-ActvSub-KO-female-NaCl 0.9856248
KO-male-NaCl-KO-female-NaCl KO-male-NaCl-KO-female-NaCl 1.0000000
WT-female-ActvSub-KO-female-NaCl WT-female-ActvSub-KO-female-NaCl 0.9676744
WT-female-NaCl-KO-female-NaCl WT-female-NaCl-KO-female-NaCl 0.9999978
WT-male-ActvSub-KO-female-NaCl WT-male-ActvSub-KO-female-NaCl 1.0000000
WT-male-NaCl-KO-female-NaCl WT-male-NaCl-KO-female-NaCl 0.9997295
KO-male-NaCl-KO-male-ActvSub KO-male-NaCl-KO-male-ActvSub 0.9937696
WT-female-ActvSub-KO-male-ActvSub WT-female-ActvSub-KO-male-ActvSub 0.5586144
WT-female-NaCl-KO-male-ActvSub WT-female-NaCl-KO-male-ActvSub 0.9440352
WT-male-ActvSub-KO-male-ActvSub WT-male-ActvSub-KO-male-ActvSub 0.9794082
WT-male-NaCl-KO-male-ActvSub WT-male-NaCl-KO-male-ActvSub 0.9996909
WT-female-ActvSub-KO-male-NaCl WT-female-ActvSub-KO-male-NaCl 0.9653593
WT-female-NaCl-KO-male-NaCl WT-female-NaCl-KO-male-NaCl 0.9999882
WT-male-ActvSub-KO-male-NaCl WT-male-ActvSub-KO-male-NaCl 1.0000000
WT-male-NaCl-KO-male-NaCl WT-male-NaCl-KO-male-NaCl 0.9999525
WT-female-NaCl-WT-female-ActvSub WT-female-NaCl-WT-female-ActvSub 0.9904299
WT-male-ActvSub-WT-female-ActvSub WT-male-ActvSub-WT-female-ActvSub 0.9628607
WT-male-NaCl-WT-female-ActvSub WT-male-NaCl-WT-female-ActvSub 0.7543825
WT-male-ActvSub-WT-female-NaCl WT-male-ActvSub-WT-female-NaCl 0.9999986
WT-male-NaCl-WT-female-NaCl WT-male-NaCl-WT-female-NaCl 0.9952281
WT-male-NaCl-WT-male-ActvSub WT-male-NaCl-WT-male-ActvSub 0.9994817

Analysis for parameter: Number of counter-clockwise spins

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Square Root Transformed (Count Data Fix).
3. Selected pathway: PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 53.136 49.5 44.023 25.250
Group 2 21 43.190 41.0 24.416 19.500
Sex female 23 53.913 49.0 42.056 22.500
Sex male 20 41.800 41.0 26.361 20.625
Genotype KO 18 36.556 39.0 20.278 15.875
Genotype WT 25 56.720 50.0 42.051 25.000
Factor ActvSub 22 52.091 49.5 37.922 17.000
Factor NaCl 21 44.286 37.0 33.786 25.500
Interaction KO-female-ActvSub 5 44.800 49.0 23.594 6.000
Interaction KO-female-NaCl 5 29.600 25.0 16.334 10.000
Interaction KO-male-ActvSub 4 47.250 50.0 22.247 10.375
Interaction KO-male-NaCl 4 24.250 25.0 13.745 10.875
Interaction WT-female-ActvSub 7 70.857 50.0 55.784 16.750
Interaction WT-female-NaCl 6 62.000 59.0 47.274 35.125
Interaction WT-male-ActvSub 6 39.500 39.5 28.431 16.500
Interaction WT-male-NaCl 6 52.167 59.5 31.884 23.250

1. Main comparisons

Summary of main comparisons

Test used: Welch Two Sample t-test

Comparison P-value
Group: 1 vs 2 0.6399028
Sex: male vs female 0.3168171
Genotype: WT vs KO 0.0864641
Factor: NaCl vs ActvSub 0.4478518

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: 3-Way ANOVA (Genotype x Sex x Mod)

Df Sum Sq Mean Sq F value Pr(>F)
Genotype 1 64.1106 64.1106 2.5912 0.1164
Sex 1 27.5360 27.5360 1.1129 0.2987
Mod 1 12.5844 12.5844 0.5086 0.4805
Genotype:Sex 1 15.4476 15.4476 0.6244 0.4348
Genotype:Mod 1 26.8795 26.8795 1.0864 0.3044
Sex:Mod 1 4.8949 4.8949 0.1978 0.6592
Genotype:Sex:Mod 1 17.3616 17.3616 0.7017 0.4079
Residuals 35 865.9567 24.7416 NA NA
Pairwise comparisons for interaction

Post-hoc used: Tukey HSD

Comparison P-value (Tukey)
KO-female-NaCl-KO-female-ActvSub KO-female-NaCl-KO-female-ActvSub 0.9966464
KO-male-ActvSub-KO-female-ActvSub KO-male-ActvSub-KO-female-ActvSub 0.9999991
KO-male-NaCl-KO-female-ActvSub KO-male-NaCl-KO-female-ActvSub 0.9783842
WT-female-ActvSub-KO-female-ActvSub WT-female-ActvSub-KO-female-ActvSub 0.9505994
WT-female-NaCl-KO-female-ActvSub WT-female-NaCl-KO-female-ActvSub 0.9985807
WT-male-ActvSub-KO-female-ActvSub WT-male-ActvSub-KO-female-ActvSub 0.9999465
WT-male-NaCl-KO-female-ActvSub WT-male-NaCl-KO-female-ActvSub 0.9999744
KO-male-ActvSub-KO-female-NaCl KO-male-ActvSub-KO-female-NaCl 0.9880562
KO-male-NaCl-KO-female-NaCl KO-male-NaCl-KO-female-NaCl 0.9999879
WT-female-ActvSub-KO-female-NaCl WT-female-ActvSub-KO-female-NaCl 0.5869717
WT-female-NaCl-KO-female-NaCl WT-female-NaCl-KO-female-NaCl 0.8810711
WT-male-ActvSub-KO-female-NaCl WT-male-ActvSub-KO-female-NaCl 0.9999576
WT-male-NaCl-KO-female-NaCl WT-male-NaCl-KO-female-NaCl 0.9618124
KO-male-NaCl-KO-male-ActvSub KO-male-NaCl-KO-male-ActvSub 0.9539206
WT-female-ActvSub-KO-male-ActvSub WT-female-ActvSub-KO-male-ActvSub 0.9908273
WT-female-NaCl-KO-male-ActvSub WT-female-NaCl-KO-male-ActvSub 0.9999565
WT-male-ActvSub-KO-male-ActvSub WT-male-ActvSub-KO-male-ActvSub 0.9991771
WT-male-NaCl-KO-male-ActvSub WT-male-NaCl-KO-male-ActvSub 1.0000000
WT-female-ActvSub-KO-male-NaCl WT-female-ActvSub-KO-male-NaCl 0.4632892
WT-female-NaCl-KO-male-NaCl WT-female-NaCl-KO-male-NaCl 0.7722673
WT-male-ActvSub-KO-male-NaCl WT-male-ActvSub-KO-male-NaCl 0.9979374
WT-male-NaCl-KO-male-NaCl WT-male-NaCl-KO-male-NaCl 0.8935620
WT-female-NaCl-WT-female-ActvSub WT-female-NaCl-WT-female-ActvSub 0.9995444
WT-male-ActvSub-WT-female-ActvSub WT-male-ActvSub-WT-female-ActvSub 0.7653825
WT-male-NaCl-WT-female-ActvSub WT-male-NaCl-WT-female-ActvSub 0.9915942
WT-male-ActvSub-WT-female-NaCl WT-male-ActvSub-WT-female-NaCl 0.9679146
WT-male-NaCl-WT-female-NaCl WT-male-NaCl-WT-female-NaCl 0.9999900
WT-male-NaCl-WT-male-ActvSub WT-male-NaCl-WT-male-ActvSub 0.9952869

Analysis for parameter: Total number of spins

Statistical Decision Pipeline:
1. Shapiro-Wilk (Normality) & Fligner-Killeen (Variance) tested.
2. Data state used: Raw data.
3. Selected pathway: PARAMETRIC.

Descriptive Statistics

Mean, Median, Standard Deviation (SD), and Quartile Deviation (QD) for raw data.
Factor Group N Mean Median SD Quartile Dev.
Group 1 22 149.182 145.0 81.221 59.000
Group 2 21 132.238 109.0 93.518 23.000
Sex female 23 163.565 140.0 93.107 51.750
Sex male 20 114.850 109.5 72.703 47.250
Genotype KO 18 112.444 110.5 64.028 42.875
Genotype WT 25 161.400 138.0 96.125 58.000
Factor ActvSub 22 147.182 115.5 98.853 40.875
Factor NaCl 21 134.333 135.0 74.010 54.000
Interaction KO-female-ActvSub 5 116.600 124.0 59.865 20.000
Interaction KO-female-NaCl 5 129.000 100.0 84.164 53.000
Interaction KO-male-ActvSub 4 87.000 104.0 43.321 19.750
Interaction KO-male-NaCl 4 112.000 104.0 76.039 55.750
Interaction WT-female-ActvSub 7 217.571 178.0 123.306 67.000
Interaction WT-female-NaCl 6 168.500 139.0 62.060 31.750
Interaction WT-male-ActvSub 6 130.667 114.0 87.790 36.250
Interaction WT-male-NaCl 6 119.500 97.5 82.646 47.875

1. Main comparisons

Summary of main comparisons

Test used: Welch Two Sample t-test

Comparison P-value
Group: 1 vs 2 0.5303231
Sex: male vs female 0.0614101
Genotype: WT vs KO 0.0518464
Factor: NaCl vs ActvSub 0.6311753

2. Sex-Genotype-Factor Interaction

Group/Interaction overall comparison

Test used: 3-Way ANOVA (Genotype x Sex x Mod)

Df Sum Sq Mean Sq F value Pr(>F)
Genotype 1 25081.1835 25081.1835 3.5281 0.0687
Sex 1 27226.9108 27226.9108 3.8299 0.0584
Mod 1 1246.7103 1246.7103 0.1754 0.6779
Genotype:Sex 1 5524.3730 5524.3730 0.7771 0.3840
Genotype:Mod 1 6227.8257 6227.8257 0.8760 0.3557
Sex:Mod 1 1996.3477 1996.3477 0.2808 0.5995
Genotype:Sex:Mod 1 415.0293 415.0293 0.0584 0.8105
Residuals 35 248815.2476 7109.0071 NA NA
Pairwise comparisons for interaction

Post-hoc used: Tukey HSD

Comparison P-value (Tukey)
KO-female-NaCl-KO-female-ActvSub KO-female-NaCl-KO-female-ActvSub 0.9999977
KO-male-ActvSub-KO-female-ActvSub KO-male-ActvSub-KO-female-ActvSub 0.9994460
KO-male-NaCl-KO-female-ActvSub KO-male-NaCl-KO-female-ActvSub 1.0000000
WT-female-ActvSub-KO-female-ActvSub WT-female-ActvSub-KO-female-ActvSub 0.4680285
WT-female-NaCl-KO-female-ActvSub WT-female-NaCl-KO-female-ActvSub 0.9687276
WT-male-ActvSub-KO-female-ActvSub WT-male-ActvSub-KO-female-ActvSub 0.9999926
WT-male-NaCl-KO-female-ActvSub WT-male-NaCl-KO-female-ActvSub 1.0000000
KO-male-ActvSub-KO-female-NaCl KO-male-ActvSub-KO-female-NaCl 0.9949077
KO-male-NaCl-KO-female-NaCl KO-male-NaCl-KO-female-NaCl 0.9999866
WT-female-ActvSub-KO-female-NaCl WT-female-ActvSub-KO-female-NaCl 0.6279978
WT-female-NaCl-KO-female-NaCl WT-female-NaCl-KO-female-NaCl 0.9934696
WT-male-ActvSub-KO-female-NaCl WT-male-ActvSub-KO-female-NaCl 1.0000000
WT-male-NaCl-KO-female-NaCl WT-male-NaCl-KO-female-NaCl 0.9999995
KO-male-NaCl-KO-male-ActvSub KO-male-NaCl-KO-male-ActvSub 0.9998723
WT-female-ActvSub-KO-male-ActvSub WT-female-ActvSub-KO-male-ActvSub 0.2411004
WT-female-NaCl-KO-male-ActvSub WT-female-NaCl-KO-male-ActvSub 0.8035457
WT-male-ActvSub-KO-male-ActvSub WT-male-ActvSub-KO-male-ActvSub 0.9918795
WT-male-NaCl-KO-male-ActvSub WT-male-NaCl-KO-male-ActvSub 0.9986987
WT-female-ActvSub-KO-male-NaCl WT-female-ActvSub-KO-male-NaCl 0.4977172
WT-female-NaCl-KO-male-NaCl WT-female-NaCl-KO-male-NaCl 0.9649919
WT-male-ActvSub-KO-male-NaCl WT-male-ActvSub-KO-male-NaCl 0.9999671
WT-male-NaCl-KO-male-NaCl WT-male-NaCl-KO-male-NaCl 0.9999999
WT-female-NaCl-WT-female-ActvSub WT-female-NaCl-WT-female-ActvSub 0.9635324
WT-male-ActvSub-WT-female-ActvSub WT-male-ActvSub-WT-female-ActvSub 0.5904292
WT-male-NaCl-WT-female-ActvSub WT-male-NaCl-WT-female-ActvSub 0.4401793
WT-male-ActvSub-WT-female-NaCl WT-male-ActvSub-WT-female-NaCl 0.9932884
WT-male-NaCl-WT-female-NaCl WT-male-NaCl-WT-female-NaCl 0.9703510
WT-male-NaCl-WT-male-ActvSub WT-male-NaCl-WT-male-ActvSub 0.9999979